Description
Recombination Immunoblot Kit for Antibody to Hepatitis C Virus
(Recombination Immunoblot Assay)
Nitrocellulose strips are coated with synthetic recombinant HCV antigens (CoreNS3NS4NS5) control protein.The strips are submerged in the diluted patient’s serum or plasma sample. During the incubation stepthe specific HCV antibodies will be captured on the strips. The strips are then washed to remove unbound serum proteins. Anti-human IgG antibodies conjugated to the enzyme alkaline phosphatase (AP-Conjugate) are then addedduring the second incubationthey will bind to the captured antibodies. The strips are then washed to remove unbound conjugatesubstrate solution is added to the reaction tray. After stopping the reactionthe results can be interpreted according to the brands showed on the strip.
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